- Title
- Aggregatibacter is inversely associated with inflammatory mediators in sputa of patients with chronic airway diseases and reduces inflammation in vitro
- Creator
- Goeteyn, Ellen; Taylor, Steven L.; Rogers, Geraint B.; Crabbé, Aurélie; Dicker, Alison; Bollé, Laura; Wauters, Merel; Joossens, Marie; Van Braeckel, Eva; Simpson, Jodie L.; Burr, Lucy; Chalmers, James D.
- Relation
- NHMRC.APP2008625 https://purl.org/au-research/grants/nhmrc/2028625
- Relation
- Respiratory Research Vol. 25, no. 368
- Publisher Link
- http://dx.doi.org/10.1186/s12931-024-02983-z
- Publisher
- Biomed Central (BMC)
- Resource Type
- journal article
- Date
- 2024
- Description
- Background: Chronic airway disease (CAD) is characterized by chronic airway inflammation and colonization of the lungs by pro-inflammatory pathogens. However, while various other bacterial species are present in the lower airways, it is not fully understood how they influence inflammation. We aimed to identify novel anti-inflammatory species present in lower airway samples of patients with CAD. Methods: Paired sputum microbiome and inflammatory marker data of adults with CAD across three separate cohorts (Australian asthma and bronchiectasis, Scottish bronchiectasis) was analyzed using Linear discriminant analysis Effect Size (LEfSE) and Spearman correlation analysis to identify species associated with a low inflammatory profile in patients. Results: We identified the genus Aggregatibacter as more abundant in patients with lower levels of airway inflammatory markers in two CAD cohorts (Australian asthma and bronchiectasis). In addition, the relative abundance of Aggregatibacter was inversely correlated with sputum IL-8 (Australian bronchiectasis) and IL-1β levels (Australian asthma and bronchiectasis). Subsequent in vitro testing, using a physiologically relevant three-dimensional lung epithelial cell model, revealed that Aggregatibacter spp. (i.e. A. actinomycetemcomitans, A. aphrophilus) and their cell-free supernatant exerted anti-inflammatory activity without influencing host cell viability. Conclusions: These findings suggest that Aggregatibacter spp. might act to reduce airway inflammation in CAD patients.
- Subject
- Chronic airway disease (CAD); bacterial species; inflammation; patients; SDG 3; Sustainable Development Goal
- Identifier
- http://hdl.handle.net/1959.13/1516751
- Identifier
- uon:57022
- Identifier
- ISSN:1465-9921
- Rights
- This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.
- Language
- eng
- Full Text
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